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Multiplexing SS - 2 Pages

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Catalogue excerpts

AcroPrep™ Advance Filter Plates for Multiplexing Assays Superior bead recovery and low levels of false positives ensure assay reproducibility Smooth well wall – Provides efficient bead recovery, ensuring reproducible results lot after lot. High performance membrane – Does not trap microspheres in the membrane matrix. Low levels of false positives – Features Supor® membrane with low non-specific binding properties that minimize non-specific reactivity by the microspheres. Plate redesign – Results in faster and more uiniform filtration rates across the plate. New outlet tip geometry – Minimizes sample leakage and loss during incubation steps so that acquisition times are not effected. Low non-specific binding – Intrinsic plate and membrane properties prevent target analytes from binding and interfering with assay performance. 100% visual inspection – Ensures each well has proper membrane placement and is free from debris. Automation compatible – Manufactured in accordance with SBS guidelines, allowing plates to be run in manual, semi-automated, and automated processes. Applications Bead-based multiplexing assays Flow cytometry

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Figure 3 Pall Supor Membrane Reduces the Occurrence of False Positive Results Performance Figure 1 Supor Membrane Does Not Trap Microspheres and Allows Efficient Bead Recovery B: Competitor M membrane The consistent membrane structure and smooth surface morphology of Pall’s Supor membrane provides efficient recovery of microspheres. The fibrous surface structure of the Competitor M membrane entraps microspheres, making recovery difficult. Luminex xMAPN microspheres were coated with a BSA solution and then diluted in PBS with 0.1% BSA to 50,000 beads per mL. Images taken following filtration. A:...

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